[ACVM] To heat inactivate or not heat inactivate ... that's the question

[Jerry Saliki]

Dear Steve et al:



In addition to the article cited by Matt Sylte, please read the article below. Based on this and other readily available scientific information, I now believe it is a waste of time to heat inactivate FBS.



https://www.yumpu.com/en/document/view/51736568/heat-inactivation-are-you-wasting-your-time-thermo-scientific.



________________________________________

Jeremiah T. Saliki, DVM, PhD, DACVM

Professor and Director

Athens Veterinary Diagnostic Laboratory

University of Georgia, Athens GA 30602

Tel: 706-542-5568

Website: www.ugavetlab.org<http://www.ugavetlab.org>



Reminder: This message and any responses to it constitute public records that may be made publicly available to anyone who requests them.



-----Original Message-----

From: ACVM_diplomates [mailto:acvm_diplomates-bounces at lists.osu.edu] On Behalf Of Krakowka, George

Sent: Tuesday, June 14, 2016 3:24 PM

To: ACVM - The American College of Veterinary Microbiologists Listserv; discussion at aavld.org<mailto:discussion at aavld.org>

Subject: Re: [ACVM] To heat inactivate or not heat inactivate ... that's the question



We done this since the beginning of time (1971 at least) but probably because we have always done it.  I have to believe that Heat Inactivation did some good although what I am not too sure. Regardless, I am reluctant to change this part of the SOP now in case there ARE differences between them and these are reflected in viral titrations, etc etc etc, Steve Krakowka) ________________________________________

From: ACVM_diplomates [acvm_diplomates-bounces at lists.osu.edu] on behalf of Jerry Saliki [jsaliki at uga.edu]

Sent: Tuesday, June 14, 2016 12:45 PM

To: discussion at aavld.org<mailto:discussion at aavld.org>; ACVM - The American   College of Veterinary Microbiologists Listserv

Subject: [ACVM] To heat inactivate or not heat inactivate ... that's the        question



To all of you cell culturists out there, I wonder if you still heat inactivate your fetal bovine serum prior to use in cell cultures and other assays. And if you heat inactivate, is it because it has been in your SOP forever or because you actually believe heat inactivation is beneficial?



________________________________________

Jeremiah T. Saliki, DVM, PhD, DACVM

Professor and Director

Athens Veterinary Diagnostic Laboratory

University of Georgia, Athens GA 30602

Tel: 706-542-5568

Website: www.ugavetlab.org<http://www.ugavetlab.org/<http://www.ugavetlab.org%3chttp:/www.ugavetlab.org/>>



Reminder: This message and any responses to it constitute public records that may be made publicly available to anyone who requests them.

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