[ACVM] To heat inactivate or not heat inactivate ... that's the question

[Tewari, Deepanker]

Agree, C inact. is the main reason for heat treatment at 56. More important during testing assays (e.g. SN) to stay consistent and avoid any outside influence.

Complement in presence of any abodies in serum (even low level) has been shown to activate C. Alternatively, certain pathogens are known to activate C with direct binding.

Good one Jerry,

Thanks
Deep Tewari

Sent from my Verizon Wireless 4G LTE smartphone


-------- Original message --------
From: Yan.Zhang at Agri.ohio.gov
Date: 06/14/2016 4:35 PM (GMT-05:00)
To: ACVM - The American College of Veterinary Microbiologists Listserv <acvm_diplomates at lists.osu.edu>, discussion at aavld.org
Subject: Re: [ACVM] To heat inactivate or not heat inactivate ... that's the question

I believe the heat inactivation is to inactivate the complement in the fetal bovine serum so that your assays (virus isolation, virus neutralization and etc) will not be affected.

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Yan Zhang, DVM, Ph.D, DACVM
Section Head of Virology and Molecular Diagnostics

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-----Original Message-----
From: ACVM_diplomates [mailto:acvm_diplomates-bounces at lists.osu.edu] On Behalf Of Krakowka, George
Sent: Tuesday, June 14, 2016 3:24 PM
To: ACVM - The American College of Veterinary Microbiologists Listserv; discussion at aavld.org
Subject: Re: [ACVM] To heat inactivate or not heat inactivate ... that's the question

We done this since the beginning of time (1971 at least) but probably because we have always done it.  I have to believe that Heat Inactivation did some good although what I am not too sure. Regardless, I am reluctant to change this part of the SOP now in case there ARE differences between them and these are reflected in viral titrations, etc etc etc, Steve Krakowka) ________________________________________
From: ACVM_diplomates [acvm_diplomates-bounces at lists.osu.edu] on behalf of Jerry Saliki [jsaliki at uga.edu]
Sent: Tuesday, June 14, 2016 12:45 PM
To: discussion at aavld.org; ACVM - The American   College of Veterinary Microbiologists Listserv
Subject: [ACVM] To heat inactivate or not heat inactivate ... that's the        question

To all of you cell culturists out there, I wonder if you still heat inactivate your fetal bovine serum prior to use in cell cultures and other assays. And if you heat inactivate, is it because it has been in your SOP forever or because you actually believe heat inactivation is beneficial?

________________________________________
Jeremiah T. Saliki, DVM, PhD, DACVM
Professor and Director
Athens Veterinary Diagnostic Laboratory
University of Georgia, Athens GA 30602
Tel: 706-542-5568
Website: www.ugavetlab.org<http://www.ugavetlab.org/>

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