[ACVM] To heat inactivate or not heat inactivate ... that's the question

[Krakowka, George]

We done this since the beginning of time (1971 at least) but probably because we have always done it.  I have to believe that Heat Inactivation did some good although what I am not too sure. Regardless, I am reluctant to change this part of the SOP now in case there ARE differences between them and these are reflected in viral titrations, etc etc etc, Steve Krakowka)
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From: ACVM_diplomates [acvm_diplomates-bounces at lists.osu.edu] on behalf of Jerry Saliki [jsaliki at uga.edu]
Sent: Tuesday, June 14, 2016 12:45 PM
To: discussion at aavld.org; ACVM - The American   College of Veterinary Microbiologists Listserv
Subject: [ACVM] To heat inactivate or not heat inactivate ... that's the        question

To all of you cell culturists out there, I wonder if you still heat inactivate your fetal bovine serum prior to use in cell cultures and other assays. And if you heat inactivate, is it because it has been in your SOP forever or because you actually believe heat inactivation is beneficial?

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Jeremiah T. Saliki, DVM, PhD, DACVM
Professor and Director
Athens Veterinary Diagnostic Laboratory
University of Georgia, Athens GA 30602
Tel: 706-542-5568
Website: www.ugavetlab.org<http://www.ugavetlab.org/>

Reminder: This message and any responses to it constitute public records that may be made publicly available to anyone who requests them.
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