[ACVM] To heat inactivate or not heat inactivate ... that's the question

[Sylte, Matthew - ARS]

Hi Jerry,

I don't heat inactivate FBS for cell culture due to a low amount of C3, based on the findings of Triqila and Linscott.

http://www.ncbi.nlm.nih.gov/pubmed/7432351

Matt

Matthew J. Sylte, DVM, PhD, DACVM
Research Veterinary Medical Officer
USDA-ARS National Animal Disease Center
Food Safety and Enteric Pathogens Research Unit
1920 Dayton Ave., Bldg. 20
Ames, IA  50010

Office Phone:  515-337-6828
Laboratory Phone: 515-337-7295
Fax: 515-337-6190
Website: https://www.ars.usda.gov/pandp/people/people.htm?personid=50278

-----Original Message-----
From: ACVM_diplomates [mailto:acvm_diplomates-bounces at lists.osu.edu] On Behalf Of Jerry Saliki
Sent: Tuesday, June 14, 2016 11:46 AM
To: discussion at aavld.org; ACVM - The American College of Veterinary Microbiologists Listserv
Subject: [ACVM] To heat inactivate or not heat inactivate ... that's the question

To all of you cell culturists out there, I wonder if you still heat inactivate your fetal bovine serum prior to use in cell cultures and other assays. And if you heat inactivate, is it because it has been in your SOP forever or because you actually believe heat inactivation is beneficial?

________________________________________
Jeremiah T. Saliki, DVM, PhD, DACVM
Professor and Director
Athens Veterinary Diagnostic Laboratory
University of Georgia, Athens GA 30602
Tel: 706-542-5568
Website: www.ugavetlab.org<http://www.ugavetlab.org/>

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